Top ten recommendations to validate your NGS assay.
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Essential genes are defined as genes that are critical for the survival of an organism. These are considered to be genes that are absolutely required for the cell to grown, proliferate and survive. Deletion of an essential gene from a cell eventually leads to the death of this cell or a severe proliferation defect. As a consequence, it is impossible to generate cells with a knock-out or deletion of essential genes.
Nuclease resistance improves performance of synthetic CRISPR RNA with Cas9 mRNA
Following transfection of CRISPR reagents, cells will need to be single cell diluted to obtain a clonal population. In this blog, we review the method used in-house.
A review of the main 5 tips to choose and use a cell line. We highlight the importance of checking for authenticity, and contamination.